|
Basic Local Alignment Search Tool.
BLAST Introduction
The BLAST programs are widely used tools for searching DNA and protein databases for sequence similarity to identify
homologs to a query sequence. While often referred to as just "BLAST", this can really be thought of as a
set of programs: blastp, blastn, blastx, tblastn, and tblastx.
The five flavours of BLAST perform the following tasks:
- blastp
compares an amino acid query sequence against a protein sequence database
- blastn
compares a nucleotide query sequence against a nucleotide sequence database
- blastx
compares the six-frame conceptual translation products of a nucleotide query
sequence (both strands) against a protein sequence database
- tblastn
compares a protein query sequence against a nucleotide sequence database
dynamically translated in all six reading frames (both strands).
- tblastx
compares the six-frame translations of a nucleotide query sequence against the six-frame
translations of a nucleotide sequence database. (Due to the nature of tblastx, gapped alignments
are not available with this option)
We provide access to a large number of nucleotide and protein BLAST
databases for all molbiol account holders. These databases are derived from the major publically-available
sequence databases.
Running BLAST
There are two main versions of blast currently available. These programs are very similar but can
produce slightly different results as they often run using different default parameters.
- blastall (from NCBI)
- WU-BLAST2 (from Washington University)
Note. We currently support only NCBI's, 'blastall' on our system.
External BLAST links:
|
