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Bioinformatics FAQ
There are a large number of bioinformatics programs available to CBRG account holders via this web site. These
examples (Frequently Asked Questions) are used to illustrate the use of these tools
in answering typical queries posed by researchers.
The major web-based tools from the CBRG are listed, here.
How do I run a BLAST search at the CBRG?
Program name: BLAST
Notes: The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity
between sequences. We maintain a large number of BLAST-searchable databases.
The five BLAST programs perform the following tasks:
- blastp
compares an amino acid query sequence against a protein sequence database
- blastn
compares a nucleotide query sequence against a nucleotide sequence database
- blastx
compares the six-frame conceptual translation products of a nucleotide query
sequence (both strands) against a protein sequence database
- tblastn
compares a protein query sequence against a nucleotide sequence database
dynamically translated in all six reading frames (both strands).
- tblastx
compares the six-frame translations of a nucleotide query sequence against the six-frame
translations of a nucleotide sequence database. (Due to the nature of tblastx, gapped alignments
are not available with this option)
See also: Other places to carry out BLAST searches include: EBI
and NCBI.
Further reading: BLAST background
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