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Bioinformatics FAQ
There are a large number of bioinformatics programs available to CBRG account holders via this web site. These
examples (Frequently Asked Questions) are used to illustrate the use of these tools
in answering typical queries posed by researchers.
The major web-based tools from the CBRG are listed, here.
What is the molecular weight/codon frequency of my protein?
Program name: pepstats
Example output from pepstats
Molecular weight = 138771.48 Residues = 1214
Average Residue Weight = 114.309 Charge = 20.0
Isoelectric Point = 8.5752
A280 Molar Extinction Coefficient = 100420
A280 Extinction Coefficient 1mg/ml = 0.72
Improbability of expression in inclusion bodies = 0.675
Residue Number Mole% DayhoffStat
A = Ala 72 5.931 0.690
B = Asx 0 0.000 0.000
C = Cys 15 1.236 0.426
D = Asp 80 6.590 1.198
E = Glu 99 8.155 1.359
F = Phe 35 2.883 0.801
G = Gly 60 4.942 0.588
H = His 20 1.647 0.824
I = Ile 64 5.272 1.172
K = Lys 91 7.496 1.136
L = Leu 109 8.979 1.213
M = Met 40 3.295 1.938
N = Asn 39 3.213 0.747
P = Pro 68 5.601 1.077
Q = Gln 53 4.366 1.119
R = Arg 98 8.072 1.647
S = Ser 76 6.260 0.894
T = Thr 74 6.096 0.999
V = Val 77 6.343 0.961
W = Trp 10 0.824 0.634
X = Xaa 0 0.000 0.000
Y = Tyr 34 2.801 0.824
Z = Glx 0 0.000 0.000
Property Residues Number Mole%
Tiny (A+C+G+S+T) 297 24.465
Small (A+B+C+D+G+N+P+S+T+V) 561 46.211
Aliphatic (I+L+V) 250 20.593
Aromatic (F+H+W+Y) 99 8.155
Non-polar (A+C+F+G+I+L+M+P+V+W+Y) 584 48.105
Polar (D+E+H+K+N+Q+R+S+T+Z) 630 51.895
Charged (B+D+E+H+K+R+Z) 388 31.960
Basic (H+K+R) 209 17.216
Acidic (B+D+E+Z) 179 14.745
Further reading: pepstats background
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