|
Bioinformatics FAQ
There are a large number of bioinformatics programs available to CBRG account holders via this web site. These
examples (Frequently Asked Questions) are used to illustrate the use of these tools
in answering typical queries posed by researchers.
The major web-based tools from the CBRG are listed, here.
How do I look up information about an enzyme?
Program name: redata
Notes: This program shows information about restriction enzymes including references, recognition and cleavage
sites, isoschizomers, commercial availability and methylation sensitivity (Note, does not include Type IV enzymes).
Output from redata regarding EcoRV
EcoRV
Recognition site is GATATC leaving blunt ends
Cut positions 5':3 3':3
Organism: Escherichia coli J62 pLG74
Methylated: 2(6)
Source: L.I. Glatman
Isoschizomers:
Bsc217I BshLI BsoAI Bsp16I BstRI CeqI
Eco17I Eco32I Eco178I HjaI HpyCI NanI
NflAI NsiCI Pac1110II Pfl16I Rma495II Rma496II
Rma497II SmiMII Tsp273I Uba1400I
Suppliers:
GE Healthcare (8/05)
Invitrogen Corporation (8/05)
Minotech Biotechnology (9/05)
Qbiogene (9/05)
American Allied Biochemical, Inc. (9/05)
SibEnzyme Ltd. (8/05)
Nippon Gene Co., Ltd. (8/05)
Takara Bio Inc. (9/05)
Roche Applied Science (8/05)
New England Biolabs (9/05)
Toyobo Biochemicals (9/05)
Molecular Biology Resources (8/05)
Promega Corporation (9/05)
Sigma Chemical Corporation (9/05)
Bangalore Genei (9/05)
EURx Ltd. (9/05)
CinnaGen Inc. (9/05)
References:
Baldwin, G.S., Halford, S.E., (1994) Biochem. Soc. Trans., vol. 22, pp. 300S.
Erskine, S.G., Halford, S.E., (1994) Biochem. Soc. Trans., vol. 22, pp. 299s.
Forrow, S., Lee, M., Souhami, R.L., Hartley, J.A., (1994) ACS Abstracts,
vol. 208, pp. 97.
Kholmina, G.V., Rebentish, B.A., Skoblov, Y.S., Mironov, A.A., Yankovskii,
N.K., Kozlov, Y.I., Glatmann, L.I., Moroz, A.F., Debabov, V.G., (1980)
Dokl. Akad. Nauk., vol. 253, pp. 495-497.
Nwosu, V.U., Connolly, B.A., Halford, S.E., Garnett, J., (1988) Nucleic Acids
Res., vol. 16, pp. 3705-3720.
Schildkraut, I., Banner, C.D.B., Rhodes, C.S., Parekh, S., (1984) Gene, vol.
27, pp. 327-329.
Vipond, I.B., Moon, B.J., Halford, S.E., (1994) Biochem. Soc. Trans., vol.
22, pp. 301S.
Winkler, F.K., (1994) J. Mol. Recognit., vol. 6, pp. 9.
Further reading: redata background
Back to FAQs
|
