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Bioinformatics FAQ
There are a large number of bioinformatics programs available to CBRG account holders via this web site. These
examples (Frequently Asked Questions) are used to illustrate the use of these tools
in answering typical queries posed by researchers.
The major web-based tools from the CBRG are listed, here.
Which enzymes do not cut my sequence?
Program name: remap
Notes: This program may be used to display a sequence with restriction enzymes and translation. Importantly
in this context, the display is followed by a list of enzymes that restrict the sequence (and their frequency) as well as a list of those
enzymes that did not cut within the sequence.
In addition, remap can be told to report any enzymes that cut less than a minimum number
of cuts (and/or above a maximum number of cuts) within the sequence - these values of these minimum and maximum cut-offs can be set
by the user.
Example output from remap.
NlaIV BsaXI
GsuI | Hpy178III | TstI SfeI
Eco57MI | | MwoI | | Hpy178III NspI |TspRI
\
tatattacacaaaggctccagaagccgactatcttgaagcatgtgtggtgtcagtgctac
1870 1880 1890 1900 1910 1920
----:----|----:----|----:----|----:----|----:----|----:----|
Y I T Q R L Q K P T I L K H V W C Q C Y
I L H K G S R S R L S * S M C G V S A T
Y Y T K A P E A D Y L E A C V V S V L Q
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