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Bioinformatics FAQ
There are a large number of bioinformatics programs available to CBRG account holders via this web site. These
examples (Frequently Asked Questions) are used to illustrate the use of these tools
in answering typical queries posed by researchers.
The major web-based tools from the CBRG are listed, here.
Which enzymes cut to leave a particular overhang?
Program name: restover
Notes: The EMBOSS program, "restover" takes a specified sequence and a desired cut-site overhang
sequence (for example, an overhang of CG) and searches the REBASE database for matching enzymes that create the desired
overhang sequence when they cut the input sequence. The default setting is to search for 5-prime overhangs only.
Output from restover - looking for the overhang "TCGA" in the Rhodopseudomonas palustris hemB
gene, EMBL:AB015492
# Restrict of AB015492 from 1 to 1750
#
# Minimum cuts per enzyme: 1
# Maximum cuts per enzyme: 2000000000
# Minimum length of recognition site: 2
# Number of hits with any overlap: 1123
# Base Number Enzyme Site 5' 3' [5' 3']
404 SalI GTCGAC 404 408
464 SalI GTCGAC 464 468
713 FokI GGATG 695 699
Further reading: restover background
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