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Bioinformatics FAQ
There are a large number of bioinformatics programs available to CBRG account holders via this web site. These
examples (Frequently Asked Questions) are used to illustrate the use of these tools
in answering typical queries posed by researchers.
The major web-based tools from the CBRG are listed, here.
Which enzymes cut my sequence?
Program name: restrict
Notes: The EMBOSS program, 'restrict' scans the sequence using information from
REBASE
Enzymes that recognise ambiguous sites such as XhoII (G/A G A T C C/T) are represented in the results
table with the ambiguity codes shown below:
| Ambiguity code | Nucleotides recognised |
| R | G or A (puRine) |
| Y | C or T (pYrimidine) |
| M | A or C |
| K | G or T |
| S | G or C |
| W | A or T |
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| Ambiguity code | Nucleotides recognised |
| B | C or G or T (not A) |
| D | A or G or T (not C) |
| H | A or C or T (not G) |
| V | A or C or G (not T) |
| N | A or C or G or T |
Restriction site ambiguity codes used by EMBOSS's restrict.
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Further reading: restrict background
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